Spring3d

Spring3d - suite of programs that reconstruct 3D images from segmented helical particles

• Segmentrefine3d

Program to iteratively refine 3D structure of helical specimens from segment stacks

• Segclassreconstruct

Program to compute 3D reconstruction from a single class average using a range of different helical symmetries

• Seggridexplore

Program to interactively explore grid searches according to different criteria

• Segrefine3dplot

Program to plot refinement parameters from segmentrefine3d

• Segrefine3dcyclexplore

Program to interactively explore refinement cycle statistics

• Segrefine3dgrid

Program to optimize segmentrefine3d reconstruction by varying refinement parameters systematically on a grid

• Segrefine3dinspect

Program to interactively inspect 3D reconstructions from segmentrefine3d using slice viewer

• Segclassmodel

Program to assign a series of 3D models to class averages

• Segmultirefine3d

Program to iteratively refine multiple 3D structures of helical specimens competitively from segment stacks