Spring2d

Spring2d - suite of programs that analyze two-dimensional images of helices

• Segment

Program to extract overlapping segments from micrographs

• Segmentexam

Program to examine all excised in-plane rotated segments and compute their collapsed (1D) and 2D power spectrum and width profile of helices

• Segmentclass

Program to classify excised in-plane rotated segments using SPARX’s k-means clustering

• Segmentalign2d

Program to align segments from helical specimens with a restrained in-plane rotation of 0 or 180 +/- delta degrees

• Segclassexam

Program to examine helix classes to compute their collapsed (1D) and 2D power spectrum and width profile of helices

• Segclasslayer

Program to extract amplitudes and phases from desired layer lines of class averages

• Seglayer2lattice

Program to simulate helical diffraction pattern and plot helical lattice from a series of indexed layer lines or rise/rotation parameters

• Segmentplot

Program to plot parameters from segmented helices